Exercise6: Using template searches

Use HumanMine for this exercise.

In our exploration of the PPARG gene we could see that it is Up-regulated in adipose tissue. Use the template searches in HumanMine to answer the following questions:

1. What other genes are up-regulated in adipose tissue according to the array express dataset (E-MTAB-62)? Don’t forget to add a filter for “UP” expression. Save a list of these genes.

Note: The E-MTAB-62 dataset is a human gene expression atlas of 5372 samples representing 369 different cell and tissue types, disease states and cell lines

2. Do any of the genes identified in 1. interact with PPARG? Save a list of these genes.

Need a hint? Take a look at Template Searches

Exercise6: Answers

1. What other genes are up-regulated in adipose tissue according to the Array express dataset? Don’t forget to add a filter for “UP” expression. Save a list of these genes.

Find the following template and set “Condition = Adipose tissue”

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Filter the column “Atlas Expression.Expression” for “UP” “Save as List” : Atlas Expression > Gene. You should have a list of 1, 654 genes

2. Do any of the genes identified in 1. interact with PPARG? Save a list of these genes.

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